Journal: ACS Nano

Article Title: Multifunctional Injectable Bioadhesive with Toll-like Receptor 4 and Myeloid Differentiation Factor 2 Antagonistic Anti-inflammatory Potential for Periodontal Regeneration

doi: 10.1021/acsnano.4c15922

Figure Lengend Snippet: Biocompatibility of the iCPC@MgO composite hydrogel and its effects on hPDLSCs osteogenic differentiation. (A) CCK8 assay and (B) live/dead staining for hPDLSCs’ viability after culturing on the 4 mg mL –1 hydrogel in each well. (C) Alkaline phosphatase activity assay was used to evaluate the early osteogenic abilities after culturing on 4 mg mL –1 hydrogel at 4 and 7 days. (D) ARS at 21 days of culturing on the 4 mg mL –1 hydrogel. (E) Semiquantification of calcium nodules ( n = 6 biologically independent replicates). (F,G) Western blot assay to detect the protein levels of the Wnt/β-catenin pathway at 21 days of culturing on the hydrogel. (H) Runx2, β catenin, and GSK 3β relative mRNA expression in hPDLSCs at 21 days of culturing on the hydrogel. Data are mean ± s.d. Statistical significance was analyzed by one-way ANOVA (* p < 0.05, ** p < 0.01, *** p < 0.001, ns represents nonsignificance).

Article Snippet: Human periodontal ligament cells (hPDLSCs) and human gingival fibroblasts (HGFs) were commercially obtained from the Elabscience Biotechnology Co. Ltd., Wuhan, China.

Techniques: CCK-8 Assay, Staining, ALP Activity Assay, Western Blot, Expressing

Journal: International Journal of Dentistry

Article Title: Evaluation of Osteogenic and Cementogenic Potential of Periodontal Ligament Fibroblast Spheroids Using a Three-Dimensional In Vitro Model of Periodontium

doi: 10.1155/2015/605813

Figure Lengend Snippet: The appearance of PGA and collagen on dentin after 20 days in culture. (a) Spheroid-collagen, (b) monolayer seeded collagen, and (c) spheroid-PGA (d) monolayer seeded-PDLF PGA. Red arrows show the incomplete attachment of collagen on dentin.

Article Snippet: Commercially available periodontal ligament fibroblasts (HPDLF, 2630, ScienCell Research Laboratories, CA, USA) were cultured in DMEM supplemented with 10% fetal calf serum, (Sigma-Aldrich, Poole, UK) 2 mM glutamine (Sigma-Aldrich, Poole, UK), 50 U/mL penicillin (Sigma-Aldrich, Poole, UK), and 50 U/mL streptomycin (Sigma-Aldrich, Poole, UK).

Techniques:

Journal: Molecular oral microbiology

Article Title: Characterization and development of SAPP as a specific peptidic inhibitor that targets Porphyromonas gingivalis

doi: 10.1111/omi.12246

Figure Lengend Snippet: HOKs and HPLFs were treated SAPP (0, 50, 200, and 800 μM) for 24 or 48 h. Cytotoxicity was determined by measuring LDH levels in the culture supernatant of HOKs and HPLFs (A and B). SAPP-induced cell death was quantitated using an Annexin V-FITC apoptosis kit (C and D). Each bar represents the mean ± standard deviation of LDH levels or numbers of apoptotic and necrotic cells. Data are the mean of at least three independent experiments.

Article Snippet: Human oral keratinocytes (HOKs) and human periodontal ligament fibroblasts (HPLFs) were purchased from ScienCell Research Laboratories.

Techniques: Standard Deviation